CODEX_VisiumHD
This protocol outlines the required reagents and step-by-step procedures for performing CODEX imaging followed by VisiumHD spatial transcriptomics on the same tissue section. This was originally described in the publication “Same-Slide Spatial Multi-Omics Integration Reveals Tumor Virus-Linked Spatial Reorganization of the Tumor Microenvironment”, with modifications adapted from protocols provided by Akoya Biosciences (PhenoCycler Fusion) and 10x Genomics (VisiumHD). Please cite when using this protocol.